POS-Holder

                  

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POS-Holder

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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

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Description

The POS-Holder, made from blue POM, is screwed into the copper block of the O2k-Main Unit, guiding theguiding the POS to the 2-mL O2k-chamber, and keeping the SmartPOS/OroboPOS-Connector in a fixed position for sealing the O2k-chamber with the POS-Seal Tip. In addition, the POS-Holder fixes the O2k-Chamber in an accurate rotational position by pressing against the angular cut of the glass chamber.

Two units of this item are standard components mounted on the O2k-Main Unit.

Reference: Product details and purchase information

Assembly

• Manual: MiPNet22.11_O2k-FluoRespirometer-manual.pdf Section 5.2.

• Video: Chamber service Insert O2k Chamber

A damaged POS-Holder may cause medium to leak from the O2k-Chamber

• The POS-Holder may be damaged:

1. During chamber assembly, when fixing the chamber in a wrong rotational position with the O2k-Chamber Holder (from above) before the POS-Holder is fully inserted, and subsequently screwing tightly the POS-Holder (see center picture below).
2. After fully unscrewing the POS-Holder and forcing during reassembly an improperly aligned holder into the thread of the O2k-Main Unit.

• We recommend to check before any reassembly the mechanical integrity of the POS-Holder (see pictures below).

• Left picture: brand new POS-Holder, no damage on the thread, no damage on the edge.
• Center picture: used POS-Holder, almost no damage on the thread, the edge is slightly damaged.
• Right picture: heavily used POS-Holder, the thread is slightly damaged, serious damage on the edge of the aperture.

• Replacement of a damaged POS-Holder is recommended. For ordering spare parts: Spare parts & consumables

See Discussion page

Keywords

»Bioblast links: Chamber volume - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

» Volume

• Units: Liter [L]; 1 L = 1 dm³ = 10^-3 m³; 1 mL = 1 cm³; 1 µL = 1 mm³

» O2k-chamber

» The experimental chamber in the instrumental O2k-Chamber

» O2k-Chamber sV

» Calibration of O2k-chamber volume

• On chamber volume

» O2k-chamber

» Chamber volume

» MiPNet09.01 O2k-ParadigmShift

» Cell count and normalization in HRR

» Open chamber

» Closed chamber

• Components of the experimental O2k-chambers

» Stirrer-Bar\white PVDF\15x6 mm

» Stirrer-Bar sV\white PVDF\11.5x6.2 mm

» Stopper\black PEEK\conical Shaft\central Port

» Stopper sV\black PEEK\conical Shaft\central Port

» O-ring\Viton\12.5x1 mm

» O-ring sV\Viton\9.5x1 mm

» Volume-Calibration Ring

» Volume-Calibration Ring sV

» Stopper-Spacer

» Cover-Slip\black

» O2k-Chamber Holder

» O2k-Chamber Holder sV

» OroboPOS-Holder

» OroboPOS-Holder sV

• Maintencance

» O2k-cleaning and ISS

» Quality control: O2k-chamber test

MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry