O2k-Catalogue: O2k-Modules

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O2k-Catalogue: O2k-Modules

O2k-Catalogue

Description 4#### O2k-Catalogue: O2k-Modules. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user.
Product ID 4####
Type O2k, MultiSensor
Link
Image 4####


O2k-Core to FluoRespirometer

O2k-Modules

O2k-Modules are add-on modules supported by the O2k. When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux in the O2k, this is an O2k-MultiSensor application. The O2k fully supports the O2k-Modules.

High-resolution respirometry is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H2O2 production, mitochondrial membrane potential, intracellular pH, Ca2+, Mg2+, and NADH levels.

Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:

  1. Quality control of preparations by respirometric performance.
  2. Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP+ or fluorophores.
  3. Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
  4. Additional information is acquired for a limited amount of sample.
  5. Oxygen dependence of parameters can be studied.


O2k-Catalogue: O2k-Modules

 DescriptionProduct idProduct image
TIP2k-Module
TIP2k with 200 mm3 microsyringe.JPG
TIP2k-Module - Titration-Injection microPump (TIP2k) for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat).
11100-03
O2k-sV-ModuleO2k-sV-Module:
11200-01.jpg
Included in all O2k-Packages, comprised of Duran glass chambers of 12 mm inner diameter specifically developed to perform high-resolution respirometry with reduced amounts of biological sample, and all components necessary for smaller operation volume (V) of 0.5 cm3 (0.5 mL). Current DatLab version is included in the delivery of this revolutionary module.
11200-01
O2k-Fluo LED2-Module
Fluorescence-Control Unit lettered.jpg
The O2k-Fluo LED2-Module is a component of the O2k-Fluorometer (O2k-Series D to G). It is an amperometric add-on module to the O2k-Core (O2k-Series D to G), adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex UltraRed), ATP production (Magnesium green), mt-membrane potential (Safranin, TMRM), Ca2+ (Calcium green), and numerous other applications open for O2k-user innovation.
12100-02
O2k-Fluo Smart-Module
12100-03.jpg
The O2k-Fluo Smart-Module is a component of the O2k-FluoRespirometer (O2k-Series H). It is an amperometric add-on module to the O2k-Respirometer, adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex UltraRed), ATP production (Magnesium green), mt-membrane potential (Safranin, TMRM), Ca2+ (Calcium green), and numerous other applications open for O2k-user innovation.
12100-03
O2k-TPP+ ISE-Module
TPP new.jpg
O2k-TPP+ ISE-Module: Potentiometric ion-selective electrodes for measurement of mitochondrial membrane potential
12300-03
O2k-pH ISE-Module
PH new.jpg
O2k-pH ISE-Module: two pH electrodes and reference electrodes and accessories
12400-02
O2k-NO Amp-Module
O2k-NO Amp-Module.jpg
O2k-NO Amp-Module: NO-sensor compatability pack an amperometric add-on for O2k-MultiSensor application The NO sensor is not included.
12500-02


O2k-MultiSensor Manual

ChapterSectionLast update
MiPNet15.03 O2k-MultiSensor-ISE
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).
2018-01-25
MiPNet15.05 NO-manual
O2k-Manual
O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.
2016-04-21
MiPNet17.05 O2k-Fluo LED2-Module
O2k-Manual
O2k-Fluo LED2-Module.
2016-08-09
MiPNet23.15 O2k-pH ISE-Module
O2k-Manual
Measurement of proton flux with the O2k-pH ISE-Module.
2018-09-08
 »O2k-Publications: O2k-Fluorometry

References

  • Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. - »Bioblast link«
  • Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - »Bioblast link«