O2k-Catalogue: O2k-Modules

From Bioblast
Jump to: navigation, search

Oroboros Instruments

News         O2k-Products         O2k-Purchase         O2k-Open Support         O2k-Publications         O2k-Workshops and Events         O2k-Network         O2k-Feedback         NextGen-O2k



O2k-Catalogue: O2k-Modules

O2k-Catalogue

Description 4#### O2k-Catalogue: O2k-Modules. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user.
Product ID 4####
Type O2k, MultiSensor
Link
Image 4####
O2k-Core to FluoRespirometer

O2k-Modules

O2k-Modules are add-on modules supported by the O2k. When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux in the O2k, this is an O2k-MultiSensor application. The O2k fully supports the O2k-Modules.

High-resolution respirometry is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H2O2 production, mitochondrial membrane potential, intracellular pH, Ca2+, Mg2+, and NADH levels.

Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:

  1. Quality control of preparations by respirometric performance.
  2. Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP+ or fluorophores.
  3. Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
  4. Additional information is acquired for a limited amount of sample.
  5. Oxygen dependence of parameters can be studied.


O2k-Catalogue: O2k-Modules

 DescriptionProduct idProduct image
TIP2k-ModuleTIP2k-Module - Titration-Injection microPump (TIP2k) for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat). The TIP2k-Module includes TIP2k-Accessories: 4 Microinjection syringes, 1 Pkg. TIP2k-Needle Spacers with mounting tool, 1 TIP2k-Needle Safety Support and 1 Pkg. TIP2k Filter Papers, and the newest DatLab version.11100-03
TIP2k with 200 mm3 microsyringe.JPG
O2k-sV-ModuleO2k-sV-Module: Now included in all O2k-Packages, comprised of Duran glass chambers of 12 mm inner diameter specifically developed to perform high-resolution respirometry with reduced amounts of biological sample, and all components necessary for smaller operation volume (V) of 0.5 cm3 (0.5 mL). Current DatLab version is included in the delivery of this revolutionary module.

The O2k-sV-Module is compatible with all O2k-Series and it is designed specifically for respirometry using small amounts of sample.

The O2k-sV-Module cannot be used for simultaneous measurement of multiple parameters in the O2k-FluoRespirometer or in combination with the O2k-MultiSensor Modules.
11200-01
11200-01.jpg
O2k-Fluo LED2-ModuleThe O2k-Fluo LED2-Module is a component of the O2k-Fluorometer (O2k-Series D to G). It is an amperometric add-on module to the O2k-Core (O2k-Series D to G), adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex UltraRed), ATP production (Magnesium green), mt-membrane potential (Safranin, TMRM), Ca2+ (Calcium green), and numerous other applications open for O2k-user innovation. The O2k-Fluo LED2-Module consists of optical sensors for both O2k-Chambers (LEDs for green and blue excitation), optical filters and the Fluorescence-Control Unit for regulation of light intensity and data input into the O2k-Main Unit (O2k-Series D to G). It includes the updated DatLab software.12100-02
Fluorescence-Control Unit lettered.jpg
O2k-Fluo Smart-ModuleThe O2k-Fluo Smart-Module is a component of the O2k-FluoRespirometer(O2k-Series H). It is an amperometric add-on module to the O2k-Respirometer, adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex UltraRed), ATP production (Magnesium green), mt-membrane potential (Safranin, TMRM), Ca2+ (Calcium green), and numerous other applications open for O2k-user innovation. Current DatLab version is included in the delivery of this module.12100-03
12100-03.jpg
O2k-TPP+ ISE-ModuleO2k-TPP+ ISE-Module: 2 potentiometric ion-selective electrodes (ISE), ISE-Service Kit and 2 PEEK-Stoppers, supported by the Oroboros O2k (O2k-Series D and higher). It also includes the newest DatLab Version. The ISE module is designed with replaceable membranes making it possible to measure different ions such as TPP+ or Ca2+ with the same electrode housing1. The main application of the O2k-TPP+ ISE-Module is measurement of mitochondrial membrane potential with tetraphenylphosphonium (TPP+) or TPMP+. 1 There are other ion selective membranes available (e.g., from Sigma Aldrich), for a wide variety of ionic species. If cut to a circle with a diameter of 4 mm these membranes can be used in the ISE system. Many ions may also be detected by fluorimetric based methods.12300-03
TPP new.jpg
O2k-pH ISE-ModuleO2k-pH ISE-Module: two pH electrodes and reference electrodes and accessories, two black PEEK stoppers, supported by the Oroboros O2k. It also includes the newest DatLab version. Quality control: Stability of Oroboros pH electrodes is tested for drift <15 µpH·s-1·mL-1.12400-02
PH new.jpg
O2k-NO Amp-ModuleO2k-NO Amp-Module: 2 black PEEK stoppers for amperometric O2k-MultiSensor application (NO, H2S, H2O2); 1 additional hole (2.3 mm); central titration port; conical bottom; with Volume-Calibration Rings (A and B); 2 O-rings mounted on each stopper, with 16 spare O-rings (O-ring\Viton\12.5x1 mm; two boxes). The NO sensor is not included.12500-02
O2k-NO Amp-Module.jpg


O2k-MultiSensor Manual

ChapterSectionLast update
MiPNet15.03 O2k-MultiSensor-ISE
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).
2018-01-25
MiPNet15.05 NO-manual
O2k-Manual
O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.
2016-04-21
MiPNet17.05 O2k-Fluo LED2-Module
O2k-Manual
O2k-Fluo LED2-Module.
2016-08-09
MiPNet23.15 O2k-pH ISE-Module
O2k-Manual
Measurement of proton flux with the O2k-pH ISE-Module.
2018-09-08
 »O2k-Publications: O2k-Fluorometry

References

  • Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. - »Bioblast link«
  • Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - »Bioblast link«