Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

O´Gorman 1997 FEBS Lett

From Bioblast
Publications in the MiPMap
O'Gorman E, Beutner G, Dolder M, Koretsky AP, Brdiczka D, Wallimann T (1997) The role of creatine kinase in inhibition of mitochondrial permeability transition. FEBS Lett 414:253-7.

» PMID: 9315696 Open Access

O'Gorman E, Beutner G, Dolder M, Koretsky AP, Brdiczka D, Wallimann T (1997) FEBS Lett

Abstract: Cyclosporin A sensitive swelling of mitochondria isolated from control mouse livers and from the livers of transgenic mice expressing human ubiquitous mitochondrial creatine kinase occurred in the presence of both 40 μM calcium and 5 μM atractyloside which was accompanied by a 2.5-fold increase over state 4 respiration rates. Creatine and cyclocreatine inhibited the latter only in transgenic liver mitochondria. Protein complexes isolated from detergent solubilised rat brain extracts, containing octameric mitochondrial creatine kinase, porin and the adenine nucleotide translocator, were reconstituted into malate loaded lipid vesicles. Dimerisation of creatine kinase in the complexes and exposure of the reconstituted complexes to > 200 μM calcium induced a cyclosporin A sensitive malate release. No malate release occurred with complexes containing octameric creatine kinase under the same conditions. Keywords: Adenine nucleotide translocator, Apoptosis, Creatine kinase, Cyclocreatine, Mitochondria, Permeability transition

O2k-Network Lab: CH Zurich Wallimann T, DE Konstanz Brdiczka D


Organism: Human, Mouse  Tissue;cell: Liver  Preparation: Isolated mitochondria 

Regulation: Substrate  Coupling state: OXPHOS 

HRR: Oxygraph-2k