MitoKIN

                

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MitoKIN

MitoKIN - Systematic barcoding of kinase-controlled mitochondrial function

MitoKIN: FFG BRIDGE 1

• FFG BRIDGE 1 30. Call - Project No. 877163, University of Innsbruck, Institute of Biochemistry
• Duration: 36 months extended additional 12 months
• Start: 2020-02-03

Dr. Eduard Stefan and Dr. Omar Torres-Quesada receiving the O2k instruments for the MitoKIN project.

About MitoKIN

Led by the Institute of Biochemistry at the University of Innsbruck, the MitoKIN project aims to connect specific/broad kinase activities to precise metabolic functions (mitochondria) relevant for cell proliferation or survival. Applied inhibitors could then be used to barcode involved kinase pathways that change the metabolome by boosting or preventing mitochondrial functions.

Aims

The MitoKIN project has three major aims:

1. Impact of specific/general kinase inhibition on metabolism & mitochondrial function.

2. Perturbation of PKA signaling in cancer cells (high cAMP levels).

3. Perturbation of PKA signaling in neurodegenerative disease models (low cAMP levels).

Coordinator

• Eduard Stefan, Ph.D. - Institute of Biochemistry, University of Innsbruck - https://www.uibk.ac.at/biochemistry/people/stefan/

Network

Back row, left to right: Eduard, Verana, Carolina, Erich; front row left to right: Lisa, Omar

Participants

Institute of Biochemistry, University of Innsbruck

• Eduard Stefan, Ph.D. - Project Coordinator, WP 1 & 4 Leader
• Omar Torres-Quesada, Ph.D. - Project Coordinator, WP 2-4 Leader

Oroboros Instruments GmbH - www.oroboros.at

• Erich Gnaiger, Ph.D. - PI
• Sabine Schmitt, Ph.D. - WP 2 Leader
• Verena Laner - Oroboros project management
• Lisa Tindle-Solomon - Oroboros project management

Oroboros project involvement

Oroboros Instruments will provide both infrastructure and the expertise required to conduct the respirometric measurements of the project.

The Institute of Biochemistry has been equipped with 3 O2ks for the duration of the project.

MitoKIN publications, preprints, abstracts and events

	When	Where
MiPNet25.06 IOC145 Innsbruck AT	2020-01-27	Innsbruck AT, 2020 Jan 27-29. O2k-Coaching Days - Basic IOC145.
Torres-Quesada 2022 MitoFit	2022	Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Human Plasma-Like Media fine-tune mitochondrial function and alter drug sensitivity in cancer cell lines. https://doi.org/10.26124/mitofit:2022-0008 — 2022-08-13 published in https://doi.org/10.3390/cancers14163917
Torres-Quesada 2022 MitoFit Kinase	2022	Torres-Quesada O, Strich S, Stefan E (2022) Kinase perturbations redirect mitochondrial function. https://doi.org/10.26124/mitofit:2022-0011 — 2022-11-15 published in Bioenerg Commun 2022.13.
Torres-Quesada 2022 Cancers (Basel)	2022	Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Physiological cell culture media tune mitochondrial bioenergetics and drug sensitivity in cancer cell models. https://doi.org/10.3390/cancers14163917
Torres-Quesada 2022 BEC	2022	Torres-Quesada O, Strich S, Stefan E (2022) Kinase perturbations redirect mitochondrial function in cancer. BEC 2022.13. https://doi.org/10.26124/bec:2022-0013

Links

News and articles

#SuccessStory: KinCon lässt Medikamenten-Forschung aufleuchten www.ffg.at, 2022-08-08

Krebs, Kinasen und Zellkraft­werke www.uibk.ac.at, 2020-02-04

Support

This project has received funding from the Austrian Reserach Promotion Funding Agency (FFG).