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Hogeboom 1946 J Biol Chem

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Hogeboom GH, Claude A, Hotchkiss RD (1946) The distribution of cytochrome oxidase and succinoxidase in the cytoplasm of the mammalian liver cell. J Biol Chem 165:615-29.

Β» PMID: 20276128 Open Access

Hogeboom GH, Claude A, Hotchkiss RD (1946) J Biol Chem

Abstract: See Free Text β€’ Keywords: Cytochrome oxidase, Succinoxidase, Liver cell extract


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Organism: Rat  Tissue;cell: Liver  Preparation: Homogenate  Enzyme: Complex IV;cytochrome c oxidase 



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SUMMARY

  1. A study of the distribution of two enzyme systems, cytochrome oxidase and succinoxidase, in the cytoplasm of the liver cell of the rat has been presented. A liver extract, containing only cytoplasmic material and suitable for fractionation by differential centrifugation, was utilized as an experimental tool.
  2. Both enzyme systems were shown to be associated, probably exclusively, with sedimentable granules of a relatively large size (0.5 to 2 p in diameter). A smaller sedimentable component (60 to 150 rnp in diameter), which was present in large amount in the liver extract, showed little, if any, enzyme activity and the remaining soluble material of the extract showed no activity. A number of observations suggested that the enzymatically active large granule fraction consisted chiefly of those cytoplasmic inclusions known as mitochondria.
  3. The large granules were markedly affected by changes in tonicity of the surrounding medium, their disintegration in hypotonic media being accompanied by a rapid and pronounced loss in aerobic succinoxidase activity. This decline in succinoxidase could not be explained either by loss of cytochrome oxidase activity, which remained at a high level, or, from the results of anaerobic determinations, by loss of succinic dehydrogenase itself. By repeated washing of the large granules in a hypotonic medium, it was possible, however, to reduce their anaerobic succinic dehydrogenase activity to a low level, apparently through the release of a soluble component of the enzyme system.
  4. The ingestion over a period of less than 2 months of the carcinogenic substance, p-dimethylaminoazobenzene, produced a reduction in the succinoxidase content of the large granules of rat liver.