Heim 2017 Am J Physiol Regul Integr Comp Physiol

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Heim AB, Chung D, Florant GL, Chicco AJ (2017) Tissue-specific seasonal changes in mitochondrial function of a mammalian hibernator. Am J Physiol Regul Integr Comp Physiol 313:R180-90.

» PMID: 28566305

Heim AB, Chung D, Florant GL, Chicco AJ (2017) Am J Physiol Regul Integr Comp Physiol

Abstract: Mammalian hibernators, such as golden-mantled ground squirrels (Callospermophilus lateralis; GMGS), cease to feed while reducing metabolic rate and body temperature during winter months, surviving exclusively on endogenous fuels stored before hibernation. We hypothesized that mitochondria, the cellular sites of oxidative metabolism, undergo tissue-specific seasonal adjustments in carbohydrate and fatty acid utilization to facilitate or complement this remarkable phenotype. To address this, we performed high-resolution respirometry of mitochondria isolated from GMGS liver, heart, skeletal muscle, and brown adipose tissue (BAT) sampled during summer (active), fall (prehibernation), and winter (hibernation) seasons using multisubstrate titration protocols. Mitochondrial phospholipid composition was examined as a postulated intrinsic modulator of respiratory function across tissues and seasons. Respirometry revealed seasonal variations in mitochondrial oxidative phosphorylation capacity, substrate utilization, and coupling efficiency that reflected the distinct functions and metabolic demands of the tissues they support. A consistent finding across tissues was a greater influence of fatty acids (palmitoylcarnitine) on respiratory parameters during the prehibernation and hibernation seasons. In particular, fatty acids had a greater suppressive effect on pyruvate-supported oxidative phosphorylation in heart, muscle, and liver mitochondria and enhanced uncoupled respiration in BAT and muscle mitochondria in the colder seasons. Seasonal variations in the mitochondrial membrane composition reflected changes in the supply and utilization of polyunsaturated fatty acids but were generally mild and inconsistent with functional variations. In conclusion, mitochondria respond to seasonal variations in physical activity, temperature, and nutrient availability in a tissue-specific manner that complements circannual shifts in the bioenergetic and thermoregulatory demands of mammalian hibernators.

Copyright © 2017 the American Physiological Society.

Keywords: Fatty acids, Hibernation, Metabolism, Mitochondria, Phospholipids, Respiration Bioblast editor: Kandolf G O2k-Network Lab: CA Vancouver Richards JG, US CO Fort Collins Chicco AJ


Labels: MiParea: Respiration, Comparative MiP;environmental MiP 


Organism: Other mammals  Tissue;cell: Heart, Skeletal muscle, Liver, Fat  Preparation: Isolated mitochondria 


Coupling state: LEAK, OXPHOS  Pathway: F, NS  HRR: Oxygraph-2k 

Labels, 2017-11