Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Chance 1961 J Biol Chem-III

From Bioblast
Publications in the MiPMap
Chance B, Hollunger G (1961) The interaction of energy and electron transfer reactions in mitochondria III. Substrate requirements for pyridine nucleotide reduction in mitochondria. J Biol Chem 236:1555-61.

Β» PMID: 13692278 Open Access

Chance B, Hollunger G (1961) J Biol Chem

Abstract:

  1. A specific succinate requirement for energy-linked reduction of mitochondrial pyridine nucleotide is demonstrated in pigeon heart and guinea pig kidney mitochondria.
  2. The succinate used in this reduction can be generated in the oxidation of malate plus glutamate or of Ξ±-ketoglutarate.
  3. The role of succinate is identified by the specific inhibitory responses of the reaction to malonate, phosphate, and fumarate.
  4. At least two kinds of mitochondrial pyridine nucleotide are shown to be reducible in State 4: (a) about one-third in the absence of added succinate in a malonate-insensitive reaction in the presence of a substrate such as malate plus glutamate and (b) about two-thirds in the presence of added succinate in a malonate-sensitive, energy-linked reaction. These two kinds of pyridine nucleotide may be considered to be compartmented.

β€’ Keywords: Pyridine nucleotide, Succinate

Made history


Labels:


Organism: Guinea pig, Birds  Tissue;cell: Heart, Kidney  Preparation: Isolated mitochondria  Enzyme: Complex II;succinate dehydrogenase  Regulation: Substrate  Coupling state: OXPHOS 


Made history