Soares 2017 MiPschool Obergurgl

Oxidative metabolism during hyperglycemia in individuals with obesity assessed by near infrared spectroscopy.

Link: MitoEAGLE

Soares RN, Reimer AR, Doyle-Baker PK, Murias JM (2017)

Event: MiPschool Obergurgl 2017

Acute hyperglycemia is known to upregulate mitochondrial function which in turn increases oxidative metabolism [1,2]. Obesity is associated to impaired glucose metabolism, mitochondrial dysfunction and impaired oxidative metabolism [3,4]. Then, this study aimed to non-invasively evaluate differences in oxidative metabolism in individuals with obesity compared to normal-weight using the near-infrared spectroscopy (NIRS) and vascular occlusion (VOT) technique (NIRS-VOT) during hyperglycemia.

Sixteen normal-weight individuals (BMI 21.3±1.7 kg/m²) and thirteen individuals with obesity (BMI 34.4±2.0 kg/m²) were submitted to five vascular occlusion tests (VOT) (pre, 30, 60, 90 and 120 min after glucose ingestion). Oxygen utilization was estimated from the area under the curve of the deoxyhemoglobin [HHb] signal during occlusion (AUCocc). Muscle reperfusion was derived from the area above the curve (AACrep) after cuff release.

The AUCocc of the normal-weight individuals increased from 15732 ± 2344 µM.s at Pre to 18930±3226 µM.s (P<0.05) at 90 minutes after glucose ingestion. The AUCocc decreased significantly from 14695±3341 µM.s at Pre to 11273±1825 µM.s (P<0.05) and 11360 ± 1750 µM.s (P<0.05) at 30 and 60 min, respectively, after glucose ingestion. The AUCrep decreased significantly from 6450±765 µM.s at Pre to 4830±963 µM.s (P<0.05) at 60 min and to 4210±595 µM.s (P<0.01) at 90 min in normal-weight individuals after glucose ingestion.

This study showed in vivo and noninvasively differences in the oxidative metabolism of skeletal muscle in individuals with obesity compared to their lean counterparts during hyperglycemia.

• Bioblast editor: Kandolf G

Labels: Pathology: Obesity 

Organism: Human 

Event: B2, Oral 

Affiliations

Soares RN(1), Reimer AR(1,2), Doyle-Baker PK(1,3), Murias JM(1)

1. Fac Kinesiol
2. Dept Biochem Molec Biol, Cumming School Med
3. Fac Environmental Design

Univ Calgary, Canada.- [email protected]

References ans support

1. Kelley DE, Mandarino LJ (1990) Hyperglycemia normalizes insulin-stimulated skeletal muscle glucose oxidation and storage in noninsulin-dependent diabetes mellitus. J Clin Investig 86:1999.
2. Simoneau JA, Kelley DE (1997) Altered glycolytic and oxidative capacities of skeletal muscle contribute to insulin resistance in NIDDM. J Applied Physiol 83:166-71.
3. Heilbronn LK, Gan SK, Turner N, et al. Markers of mitochondrial biogenesis and metabolism are lower in overweight and obese insulin-resistant subjects. (2007) J Clin Endocrinol Metabol 92:1467-73.
4. Lee JY, Lee DC, Im JA, Lee JW (2014) Mitochondrial DNA copy number in peripheral blood is independently associated with visceral fat accumulation in healthy young adults. Int J Endocrinol 2014:586017.

Selected mentor: Dr Ian Lanza