Mitochondrial preparations: Difference between revisions
No edit summary |
No edit summary Β |
||
(8 intermediate revisions by 2 users not shown) | |||
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=mtprep | |abbr=mtprep | ||
|description='''Mitochondrial preparations''' (mtprep) are isolated mitochondria (imt | |description='''Mitochondrial preparations''' (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized tissue (permeabilized fibers, pfi) or permeabilized cells (pce). In mtprep the plasma membranes are either removed (imt) or mechanically (thom) and chemically permeabilized (pfi), while mitochondrial functional integrity and to a large extent mt-structure are maintainedΒ in incubation media optimized to support mitochondrial physiological performance. According to this definition, submitochondrial particles (smtp) are not a mtprep, since mitochondrial structure is altered although specific mitochondrial functions are preserved. | ||
}} | }} | ||
Communicated by [[Gnaiger E]] 2011-11-15 (last edit 2022-12-11). | |||
== MitoPedia: mt-preparations == | == MitoPedia: mt-preparations == | ||
::::* [[Isolated mitochondria]], imt | ::::* [[Isolated mitochondria]], imt | ||
::::* [[Tissue homogenate]], thom | ::::* [[Tissue homogenate]], thom | ||
::::* [[Permeabilized tissue | ::::* [[Permeabilized tissue]], pti | ||
::::* [[Permeabilized muscle | ::::* [[Permeabilized muscle fibers]], pfi | ||
::::* [[Permeabilized cells]], pce | ::::* [[Permeabilized cells]], pce | ||
::::Β» Compare: [[Submitochondrial particles]], smtp | |||
:::: '''O2k-Protocols for mt-preparations:''' [[O2k-mitochondrial preparations]] | :::: '''O2k-Protocols for mt-preparations:''' [[O2k-mitochondrial preparations]] | ||
== References == | |||
{{#ask:[[Additional label::Mitochondrial preparations]] | |||
| mainlabel=Bioblast link | |||
|?Has title=Reference | |||
|?Was published in year=Year | |||
|format=broadtable | |||
|limit=5000 | |||
|offset=0 | |||
|sort=Has title | |||
|order=ascending | |||
}} | |||
{{Labeling | {{Labeling | ||
|preparations=Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate | |preparations=Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate | ||
}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Sample preparation | |||
}} | }} |
Latest revision as of 14:29, 11 December 2022
Description
Mitochondrial preparations (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized tissue (permeabilized fibers, pfi) or permeabilized cells (pce). In mtprep the plasma membranes are either removed (imt) or mechanically (thom) and chemically permeabilized (pfi), while mitochondrial functional integrity and to a large extent mt-structure are maintained in incubation media optimized to support mitochondrial physiological performance. According to this definition, submitochondrial particles (smtp) are not a mtprep, since mitochondrial structure is altered although specific mitochondrial functions are preserved.
Abbreviation: mtprep
Communicated by Gnaiger E 2011-11-15 (last edit 2022-12-11).
MitoPedia: mt-preparations
- Isolated mitochondria, imt
- Tissue homogenate, thom
- Permeabilized tissue, pti
- Permeabilized muscle fibers, pfi
- Permeabilized cells, pce
- Β» Compare: Submitochondrial particles, smtp
- O2k-Protocols for mt-preparations: O2k-mitochondrial preparations
References
Bioblast link | Reference | Year |
---|---|---|
BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al β MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
Labels:
Preparation: Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate
MitoPedia topics: Sample preparation