MitoPediaAlert2020 Archive
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
Contents
Aug 2020
- Aug-06 Th
OXPHOS-coupling efficiency (1-L/P) describes the coupling of the mitochondrial electron transfer system. This coupling control factor ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (L=P). It is suggested to use OXPHOS-coupling efficiency instead of the respiratory acceptor control ratio (RCR) to evaluate mitochondrial coupling.
Previous MitoPedia Alert 2020
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Jul 2020
- Jul-30 Th
Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:
- Jul-24 Fr
Do you know how to assess the integrity of the mitochondrial outer membrane in mitochondrial preparations? It is possible to do so using exogenously added cytochrome c:
- »Cytochrome c control factor«
- Jul-16 Th
Do you know how to select the proper fluorescence sensors and filter set?
Using different fluorescence sensors, LED and photodiode filters, the O2k-Fluo Smart-Module (or O2k-Fluo LED2-Module) allows for measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Selecting a Filter Set«
- Jul-09 Th
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?
- Jul-02 Th
Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:
- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of an uncoupler, which inhibits ET capacity,
- inhibition of ET capacity due to the use of high oligomycin (inhibitor of the ATP synthase) concentrations employed to measure LEAK respiration prior ET capacity. Information from: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link«
Jun 2020
- Jun-25 Th
Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?
- » Dithionite«
- Jun-18 Th
Did you know the Crabtree effect can affect respiration in living cells? SUIT-003 is designed to analyze this important mechanism of respiratory control.
- »SUIT-003 O2 ce D037« and »SUIT-003 O2 ce D038«
- Jun-11 Th
Did you know that some chemicals are incompatible with the Amplex UltraRed assay?
- Jun-08 Mo
Celebrating World Oceans Day
Would you like to measure the O2 flux that occurs during photosynthesis using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 NextGen-O2k project.
- »PhotoBiology«
- Jun-04 Th
Does your sample display a preference for glutamate & malate over pyruvate & malate to support NADH-linked respiration?
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates.
-»SUIT-008« and »SUIT-014«
May 2020
- May-28 Th
Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas?
- May-21 Th
As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O2 consumption and production.
-»PhotoBiology«
- May-14 Th
How can I measure ATP production with the O2k?
Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green
- »Magnesium Green«
- May-07 Th
Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it? Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present?
- Learn more about - »malate-anaplerotic pathway control state«
- DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - »SUIT-027«
- Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - »SUIT-002«
Apr 2020
- Apr-24 Fr
ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.
- Apr-21 Tu
Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
- Apr-13 Mo
Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation
- »SUIT-024«
- Apr-08 We
The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration.
- »SUIT-010«
- Apr-02 Th
Do you want to search the best SUIT protocol for your research questions?
- Use the SUITbrowser: »SUITbrowser«
Mar 2020
- Mar-16 Mo
The standardized coupling control protocol to explore the bioenergetics of living cells
DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions.
- »SUIT-003«
- Mar-11 We
Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2.
- »Blebbistatin«
- Mar-06 Fr
Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.
- Mar-03 Tu
Rapamycin - more differences or more in common with other mitObesity drugs?
- »Rapamycin«
Feb 2020
- Feb-26 We
It is essential to titrate uncouplers in multiple steps for respirometric measurement of ET capacity.
- Feb-24 Mo
- Feb-19 We
Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.
- »Spermidine«
- Feb-18 Tu
Why do we fight gas bubbles?
- Dive deep into »Oxygen solubility«
- Feb-12 We
What do metformin, melatonin and other bioactive mitObesity compounds have in common?
- »MitObesity drugs«
Jan 2020
- Jan-16 Th
Oxygen flux
- which SI units should be used?
Did you ever think that units for body mass could be an issue?
- »Body mass«
- Jan-08 We