Difference between revisions of "Gnaiger 2014 Abstract MiP2014"

Revision as of 23:48, 26 August 2014

Cell ergometry: OXPHOS and ETS coupling efficiency.

Link:

Mitochondr Physiol Network 19.13 - MiP2014

Gnaiger E (2014)

Event: MiP2014

Biochemical cell ergometry aims at measurement of J_O2,max (compare V_O2,max in exercise ergometry of humans and animals) of cell respiration linked to phosphorylation of ADP to ATP. The corresponding OXPHOS capacity is based on saturating concentrations of ADP, [ADP]*, and inorganic phosphate, [Pi]*, available to the mitochondria. This is metabolically opposite to uncoupling respiration, which yields ETS capacity. The OXPHOS state can be established experimentally by selective permeabilization of cell membranes with maintenance of intact mitochondria, titrations of ADP and P_i to evaluate kinetically saturating conditions, and establishing fuel substrate combinations which reconstitute physiological TCA cycle function.

Labels: MiParea: Respiration

Coupling state: OXPHOS

HRR: Oxygraph-2k Event: A4, Oral MiP2014

Affiliation

1-Daniel Swarovski Research Lab, Mitochondrial Physiol, Dep Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck; 2-OROBOROS INSTRUMENTS, Innsbruck, Austria. - [email protected]

References and acknowledgements

Supported by K-Regio project MitoCom Tyrol.

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.

Figure 1: Phosphorylation control protocol in the intact cell

>> Cell ergometry >> High-resolution pdf: »File:OROBOROS Poster HRR.pdf

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@@ Line 1: / Line 1: @@
 {{Abstract
-|title=Cell ergometry, efficiency and respiratory control factors: limitation of measurements, terminology and concepts.
+|title=Cell ergometry: OXPHOS and ETS coupling efficiency.
 |info=[[File:Gnaiger Erich.jpg|120px|right|Gnaiger E]] [[Laner 2014 Mitochondr Physiol Network MiP2014|Mitochondr Physiol Network 19.13]] - [http://www.mitophysiology.org/index.php?mip2014 MiP2014]
 |authors=Gnaiger E
@@ Line 27: / Line 27: @@
 [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
-# [[Biochemical coupling efficiency]]: ''j<sub>E-L</sub>'' = (''E-L'')/''E''
-# [[ETS excess factor over R |ETS excess factor over ''R'']]: ''Ex<sub>R</sub>/E'' = (''E-R'')/''E''
-# [[ROUTINE phosphorylation control factor]]: ''≈R/R'' = (''R-L'')/''R''
-# [[ROUTINE phosphorylation control ratio]]: ''≈R/E'' = (''R-L'')/''E''