From Bioblast
Description
Saponin is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for permeabilization of muscle fibres.
Abbreviation: Sap
MitoPedia topics:
Permeabilization agent
Application in HRR
Sap: Saponin (from quillaja bark); Sigma S 2149, 25 g, store at R.T.; FW = 1229.31
Caution: Prepare fresh everyday!
Comment: The product is withdrawn from Sigma!
Preparation of 5 mg/ml stock solution (dissolved in H2O):
- 1) weigh 5 mg of Saponin
- 2) dissolve in 1 ml H2O
β use 50 Β΅g Saponin/ml BIOPS: add 20 Β΅l of 5 mg/ml saponin stock solution per 2 ml BIOPS