Dobson 1988 Am J Physiol: Difference between revisions
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{{Publication | {{Publication | ||
|title=Dobson GP, Parkhouse WS, Weber JM, Stuttard E, Harman J, Snow DH, Hochachka PW (1988) Metabolic changes in skeletal muscle and blood of greyhounds during 800-m track sprint. Am J Physiol 255: R513-R519. ย | |title=Dobson GP, Parkhouse WS, Weber JM, Stuttard E, Harman J, Snow DH, Hochachka PW (1988) Metabolic changes in skeletal muscle and blood of greyhounds during 800-m track sprint. Am J Physiol 255: R513-R519. ย | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/3414847 PMID: 3414847] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/3414847 PMID: 3414847 Open Access] | ||
|authors=Dobson GP, Parkhouse WS, Weber JM, Stuttard E, Harman J, Snow DH, Hochachka PW | |authors=Dobson GP, Parkhouse WS, Weber JM, Stuttard E, Harman J, Snow DH, Hochachka PW | ||
|year=1988 | |year=1988 |
Revision as of 15:21, 28 October 2013
Dobson GP, Parkhouse WS, Weber JM, Stuttard E, Harman J, Snow DH, Hochachka PW (1988) Metabolic changes in skeletal muscle and blood of greyhounds during 800-m track sprint. Am J Physiol 255: R513-R519. |
Dobson GP, Parkhouse WS, Weber JM, Stuttard E, Harman J, Snow DH, Hochachka PW (1988) Am J Physiol
Abstract: The aim of this study was to examine some metabolic properties and changes that occur in skeletal muscle and blood of greyhounds after an 800-m sprint. Three prime moving fast-twitch muscles were selected: biceps femoris (BF), gastrocnemius (G), and vastus lateralis (VL). The amount of glycogen utilized during the event was 42.57, 43.86, and 42.73 mumol glucosyl units/g wet wt, respectively. Expressed as a function of race time (48.3 +/- 0.7 s, n = 3), the mean rate of glycogen breakdown was 53.48 +/- 0.5 mumol.g wet wt-1.min-1 during the sprint. This is equivalent to an ATP turnover of 160 mumol.g wet wt-1.min-1, assuming 100% anaerobic conversion to lactate. This represents a conservative estimate, since greyhound muscle is heterogeneous and comprised of a large percentage of fast-twitch oxidative fibers (Armstrong et al., Am. J. Anat. 163: 87-98, 1982). The large decrease in muscle glycogen was accompanied by a 6- to 7-fold increase in muscle lactate from 3.48 +/- 0.13 to 25.42 +/- 3.54 (BF), 2.54 +/- 1.05 to 18.96 +/- 2.60 (G), and 4.57 +/- 0.44 to 30.09 +/- 1.94 mumol.g wet wt (VL), and a fall in muscle pH from 6.88 +/- 0.03 to 6.40 +/- 0.02 (BF), 6.92 +/- 0.02 to 6.56 +/- 0.02 (G), and 6.93 +/- 0.02 to 6.47 +/- 0.01 (VL). Cytosolic phosphorylation potential in BF decreased 10-fold from 11,360 +/- 680 to 1,184 +/- 347, and redox potential decreased 5-fold, indicating a marked reduction in the cytosol at this time.
Labels: MiParea: Comparative MiP;environmental MiP, Exercise physiology;nutrition;life style
Organism: Dog
Tissue;cell: Skeletal muscle
Preparation: Intact Organism"Intact Organism" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Substrate