Hagl 2015 Molecules: Difference between revisions
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|abstract=Mitochondria are involved in the aging processes that ultimately lead to neurodegeneration and the development of Alzheimer's disease (AD). A healthy lifestyle, including a diet rich in antioxidants and polyphenols, represents one strategy to protect the brain and to prevent neurodegeneration. We recently reported that a stabilized hexanic rice bran extract (RBE) rich in vitamin E and polyphenols (but unsuitable for human consumption) has beneficial effects on mitochondrial function in vitro and in vivo (doi:10.1016/j.phrs.2013.06.008, 10.3233/JAD-132084). To enable the use of RBE as food additive, a stabilized ethanolic extract has been produced. Here, we compare the vitamin E profiles of both extracts and their effects on mitochondrial function (ATP concentrations, mitochondrial membrane potential, mitochondrial respiration and mitochondrial biogenesis) in PC12 cells. We found that vitamin E contents and the effects of both RBE on mitochondrial function were similar. Furthermore, we aimed to identify components responsible for the mitochondria-protective effects of RBE, but could not achieve a conclusive result. ฮฑ-Tocotrienol and possibly also ฮณ-tocotrienol, ฮฑ-tocopherol and ฮด-tocopherol might be involved, but hitherto unknown components of RBE or a synergistic effect of various components might also play a role in mediating RBE's beneficial effects on mitochondrial function. | |abstract=Mitochondria are involved in the aging processes that ultimately lead to neurodegeneration and the development of Alzheimer's disease (AD). A healthy lifestyle, including a diet rich in antioxidants and polyphenols, represents one strategy to protect the brain and to prevent neurodegeneration. We recently reported that a stabilized hexanic rice bran extract (RBE) rich in vitamin E and polyphenols (but unsuitable for human consumption) has beneficial effects on mitochondrial function in vitro and in vivo (doi:10.1016/j.phrs.2013.06.008, 10.3233/JAD-132084). To enable the use of RBE as food additive, a stabilized ethanolic extract has been produced. Here, we compare the vitamin E profiles of both extracts and their effects on mitochondrial function (ATP concentrations, mitochondrial membrane potential, mitochondrial respiration and mitochondrial biogenesis) in PC12 cells. We found that vitamin E contents and the effects of both RBE on mitochondrial function were similar. Furthermore, we aimed to identify components responsible for the mitochondria-protective effects of RBE, but could not achieve a conclusive result. ฮฑ-Tocotrienol and possibly also ฮณ-tocotrienol, ฮฑ-tocopherol and ฮด-tocopherol might be involved, but hitherto unknown components of RBE or a synergistic effect of various components might also play a role in mediating RBE's beneficial effects on mitochondrial function. | ||
|keywords=Mitochondria, Rice bran extract, Vitamin E, Tocopherol, Tocotrienol, PC12 cells, Aging, Prevention, Neurodegeneration | |keywords=Mitochondria, Rice bran extract, Vitamin E, Tocopherol, Tocotrienol, PC12 cells, Aging, Prevention, Neurodegeneration | ||
|mipnetlab=DE Frankfurt Eckert GP | |mipnetlab=DE Giessen Eckert GP, DE Frankfurt Eckert GP | ||
}} | }} | ||
{{Labeling | {{Labeling |
Latest revision as of 18:57, 30 November 2017
Hagl S, Berressem D, Bruns B, Sus N, Frank J, Eckert GP (2015) Beneficial Effects of Ethanolic and Hexanic Rice Bran Extract on Mitochondrial Function in PC12 Cells and the Search for Bioactive Components. Molecules 20(9):16524-39. |
Hagl S, Berressem D, Bruns B, Sus N, Frank J, Eckert GP (2015) Molecules
Abstract: Mitochondria are involved in the aging processes that ultimately lead to neurodegeneration and the development of Alzheimer's disease (AD). A healthy lifestyle, including a diet rich in antioxidants and polyphenols, represents one strategy to protect the brain and to prevent neurodegeneration. We recently reported that a stabilized hexanic rice bran extract (RBE) rich in vitamin E and polyphenols (but unsuitable for human consumption) has beneficial effects on mitochondrial function in vitro and in vivo (doi:10.1016/j.phrs.2013.06.008, 10.3233/JAD-132084). To enable the use of RBE as food additive, a stabilized ethanolic extract has been produced. Here, we compare the vitamin E profiles of both extracts and their effects on mitochondrial function (ATP concentrations, mitochondrial membrane potential, mitochondrial respiration and mitochondrial biogenesis) in PC12 cells. We found that vitamin E contents and the effects of both RBE on mitochondrial function were similar. Furthermore, we aimed to identify components responsible for the mitochondria-protective effects of RBE, but could not achieve a conclusive result. ฮฑ-Tocotrienol and possibly also ฮณ-tocotrienol, ฮฑ-tocopherol and ฮด-tocopherol might be involved, but hitherto unknown components of RBE or a synergistic effect of various components might also play a role in mediating RBE's beneficial effects on mitochondrial function. โข Keywords: Mitochondria, Rice bran extract, Vitamin E, Tocopherol, Tocotrienol, PC12 cells, Aging, Prevention, Neurodegeneration
โข O2k-Network Lab: DE Giessen Eckert GP, DE Frankfurt Eckert GP
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Organism: Rat
Tissue;cell: Nervous system, Other cell lines
Preparation: Permeabilized cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET
Pathway: N, S, CIV, NS, ROX
HRR: Oxygraph-2k
PC12 cells