Gnaiger 2014 Abstract MiP2014: Difference between revisions
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== Figure 1: Phosphorylation control protocol in the intact cell == | == Figure 1: Phosphorylation control protocol in the intact cell == | ||
>> [[Cell ergometry]] | >> [[Cell ergometry]] | ||
[[File:OROBOROS Poster HRR.pdf]] | |||
[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]] | [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]] | ||
Revision as of 23:50, 26 August 2014
| Cell ergometry: OXPHOS and ETS coupling efficiency. |
Link:
Mitochondr Physiol Network 19.13 - MiP2014
Gnaiger E (2014)
Event: MiP2014
Biochemical cell ergometry aims at measurement of JO2,max (compare VO2,max in exercise ergometry of humans and animals) of cell respiration linked to phosphorylation of ADP to ATP. The corresponding OXPHOS capacity is based on saturating concentrations of ADP, [ADP]*, and inorganic phosphate, [Pi]*, available to the mitochondria. This is metabolically opposite to uncoupling respiration, which yields ETS capacity. The OXPHOS state can be established experimentally by selective permeabilization of cell membranes with maintenance of intact mitochondria, titrations of ADP and Pi to evaluate kinetically saturating conditions, and establishing fuel substrate combinations which reconstitute physiological TCA cycle function.
Labels: MiParea: Respiration
Coupling state: OXPHOS
HRR: Oxygraph-2k Event: A4, Oral MiP2014
Affiliation
1-Daniel Swarovski Research Lab, Mitochondrial Physiol, Dep Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck; 2-OROBOROS INSTRUMENTS, Innsbruck, Austria. - [email protected]
References and acknowledgements
Supported by K-Regio project MitoCom Tyrol.
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.
Figure 1: Phosphorylation control protocol in the intact cell
>> Cell ergometry File:OROBOROS Poster HRR.pdf
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