Startup O2k-Respirometer: Difference between revisions
No edit summary |
No edit summary |
||
Line 7: | Line 7: | ||
|product id=10000-02 | |product id=10000-02 | ||
|product type=O2k, O2k-Module, O2k-Fluorometer, Catalogue | |product type=O2k, O2k-Module, O2k-Fluorometer, Catalogue | ||
|info=[[Oxygraph-2k| O2k] | |info=[[Oxygraph-2k| O2k]], [[Gnaiger_2008_POS]] | ||
|product image=[[Image:O2k-Core-Concept.jpg|200px|right]] | |product image=[[Image:O2k-Core-Concept.jpg|200px|right]] | ||
}} | }} |
Revision as of 14:49, 3 April 2017
Startup O2k-Respirometer
Description | OROBOROS O2k-Core - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Core includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System and the O2k-Titration Set. The O2k-Core supports all add-on O2k-Modules of the O2k, and is a component of the O2k-Fluorometer.
On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ยฑ0.002 ยฐC in the range of 4 ยฐC to 47 ยฐC at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software. The O2k is a sole source apparatus with no other instruments meeting its specifications. |
---|---|
Product ID | 10000-02 |
Type | O2k, O2k-Module, O2k-Fluorometer, Catalogue |
Link | O2k, Gnaiger_2008_POS |
Image |
- The O2k-Core does not include, but fully supports and is required for the add-on Modules of the O2k, such as the TIP2k, O2k-Fluo LED2-Module, O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module.
- A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see here.
The O2k-Core consists of
O2k-Core manual and selected protocols
Chapter | View | Section | Last update |
---|---|---|---|
Gnaiger 2008 POS | Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52. | 2008 | |
Gnaiger 2020 BEC MitoPathways | published online 2020-12-30
| Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | 2020 |
MiPNet04.05 Titration-Injection | From step titration to ramp injection: Uncoupling by FCCP with TIP. | 1999-01 | |
MiPNet07.08 User information | ยป Versions | O2k-Manual: user information. PLEASE STUDY THIS MANUAL. | 2017-11-03 |
MiPNet08.09 CellRespiration | ยปVersions | High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX. | 2016-08-11 |
MiPNet08.12 IOC22 | NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report. | 2011-12-11 | |
MiPNet12.01 Suppl T-issue | ยปVersions | MitoPathways at the Q-junction: mouse skeletal muscle fibers. | 2018-11-18 |
MiPNet12.11 MitoRespiration | ยปVersions | Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control. | 2014-02-20 |
MiPNet17.03 Shredder vs Fibers | ยปVersions | Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder. | 2015-06-18 |
MiPNet19.18 O2k-Series G Core manual | ยปVersions | O2k-Core manual contents. | 2016-08-08 |
MiPNet19.18B POS-service | ยปVersions | Service of the polarographic oxygen sensor OroboPOS. | 2021-06-23 |
MiPNet19.18C DatLab 6: Guide | ยปVersions | DatLab-guide through the menus. | 2016-08-24 |
MiPNet19.18D O2k-Series G and DatLab 6: Calibration | ยปVersions | O2k-calibration by DatLab 6. | 2016-08-24 |
MiPNet19.18E O2 flux analysis - DatLab 6 | ยปVersions | Oxygen flux analysis: DatLab real-time. | 2016-08-08 |
MiPNet21.14 Reference sample HRR | ยปVersions | Development of a reference sample for HRR. | 2016-10-19 |
MiPNet21.16 DatLab 7 Innovations | DatLab 7: innovations. | 2019-09-23 | |
MiPNet22.16 DatLab 7 Innovations: DL-Protocols | ยป Versions | DatLab 7.4 Innovations: DL-Protocols | 2019-09-13 |
MiPNet23.12 DL-Protocols help | ยป Versions | DatLab Protocols: Help DatLab 7. | 2019-09-13 |
MiPNet24.06 Oxygen flux analysis - DatLab 7.4 | ยป Versions | Oxygen flux analysis with DatLab 7.4. | 2020-04-16 |
MiPNet24.08 Safranin Analysis Template | ยป Versions | Excel template for safranin data analysis. | 2020-05-13 |
MiPNet24.09 Data analysis of mt-membrane potential | ยป Versions | Data analysis of mt-membrane potential. | 2021-09-16 |
MiPNet25.14 TPP Analysis Template | ยป [https:.pdf Versions] | Excel template for TPP data analysis. | 2020-##-## |
MiPNet26.06 DatLab 7: Guide | ยปVersions | DatLab-guide through the menus. | 2021-06-02 |
MiPNet28.10 SmartPOS-service | ยป Versions | SmartPOS: Service | 2023-09-29 |
Pesta 2012 Methods Mol Biol | PMID: 22057559 | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 | 2012 |
MitoPedia O2k and high-resolution respirometry:
O2k hardware