Rotenone: Difference between revisions
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'''Preparation of 1 mM stock solution''' (dissolved in 99.9% | '''Preparation of 1 mM stock solution''' (dissolved in 99.9% ethanol): | ||
::1 | ::1. Weigh 0.39 mg of rotenone and dissolve in 1 ml of ethanol absolute (99.9%). | ||
::2 | ::2. Divide into 100 ยตl aliquots (use dark glass vials). | ||
::3) | ::3) Store frozen at -20 ยฐC. | ||
'''Oxygraph-2k | '''Oxygraph-2k manual titrations:'''ย [[MiPNet09.12]] | ||
ย | |||
::* Titration volume: 1 ยตl using a 10 ยตl syringe. | |||
::* Final conc. in 2 ml O2k-Chamber: 0.5 ยตM. | |||
== Inhibition of CI by rotenone == | == Inhibition of CI by rotenone == | ||
* [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724837/ Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787: 384โ392.] | * [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724837/ Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787: 384โ392.] |
Revision as of 17:35, 6 March 2014
Description
Rotenone is an (synonym. 2R,6aS,12aS)-1,2,6,6a,12,12a-hexahydro-2-isopropenyl-8,9 dimethoxychromeno[3,4-b]furo(2,3-h)chromen-6-on) inhibitor of complex I via inhibition of NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also S(Rot).
Abbreviation: Rot
Reference: Gnaiger 2012 MitoPathways, MiPNet09.12, MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Rot: Rotenone (Rotenone โฅ 95%, C23H22O6); Sigma R 8875, 1 g, store at R.T.; FW = 394.42
Caution: Very toxic and light sensitive (store solution in a dark glass vial). Use fresh aliquots for every experimental day!
Preparation of 1 mM stock solution (dissolved in 99.9% ethanol):
- 1. Weigh 0.39 mg of rotenone and dissolve in 1 ml of ethanol absolute (99.9%).
- 2. Divide into 100 ยตl aliquots (use dark glass vials).
- 3) Store frozen at -20 ยฐC.
Oxygraph-2k manual titrations: MiPNet09.12
- Titration volume: 1 ยตl using a 10 ยตl syringe.
- Final conc. in 2 ml O2k-Chamber: 0.5 ยตM.