Colosio 2023 J Appl Physiol (1985): Difference between revisions
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{{Publication | {{Publication | ||
|title=Colosio M, Brocca L, Gatti M, Neri M, Crea E, Cadile F, Canepari M, Pellegrino MA, Polla B, Porcelli S, Bottinelli R (2023) Structural and functional impairments of skeletal muscle in patients with post-acute sequelae of SARS-CoV-2 infection. https://doi.org/10.1152/japplphysiol.00158.2023 | |title=Colosio M, Brocca L, Gatti M, Neri M, Crea E, Cadile F, Canepari M, Pellegrino MA, Polla B, Porcelli S, Bottinelli R (2023) Structural and functional impairments of skeletal muscle in patients with post-acute sequelae of SARS-CoV-2 infection. https://doi.org/10.1152/japplphysiol.00158.2023 | ||
|info=J Appl Physiol (1985) | |info=J Appl Physiol (1985) 135:4. [https://pubmed.ncbi.nlm.nih.gov/37675472 PMID: 37675472 Open Access] | ||
|authors=Colosio | |authors=Colosio Marta, Brocca Lorenza, Gatti Marco, Neri Marianna, Crea Emanuela, Cadile Francesca, Canepari Monica, Pellegrino Maria Antonietta, Polla Biagio, Porcelli Simone, Bottinelli Roberto | ||
|year=2023 | |year=2023 | ||
|journal=J Appl Physiol (1985) | |journal=J Appl Physiol (1985) | ||
|abstract=Following acute COVID-19, a substantial proportion of patients showed symptoms and sequelae for several months, namely the post-acute sequelae of COVID-19 (PASC) syndrome. Major phenomena are exercise intolerance, muscle weakness and fatigue. We aimed to investigate the physiopathology of exercise intolerance in patients with PASC syndrome by structural and functional analyses of skeletal muscle. | |abstract=Following acute COVID-19, a substantial proportion of patients showed symptoms and sequelae for several months, namely the post-acute sequelae of COVID-19 (PASC) syndrome. Major phenomena are exercise intolerance, muscle weakness and fatigue. We aimed to investigate the physiopathology of exercise intolerance in patients with PASC syndrome by structural and functional analyses of skeletal muscle. | ||
At least 3 months after infection, non-hospitalized patients with PASC (n=11,ys:54ยฑ11; PASC) and patients without long-term symptoms (n=12,ys:49ยฑ9; CTRL) visited the laboratory on four non-consecutive days. Spirometry, lung diffusion capacity and quality of life were assessed at rest. Cardiopulmonary incremental exercise test was performed. Oxygen consumption ( | At least 3 months after infection, non-hospitalized patients with PASC (n=11,ys:54ยฑ11; PASC) and patients without long-term symptoms (n=12,ys:49ยฑ9; CTRL) visited the laboratory on four non-consecutive days. Spirometry, lung diffusion capacity and quality of life were assessed at rest. Cardiopulmonary incremental exercise test was performed. Oxygen consumption (VO<sub>2</sub>) kinetics were determined by moderate-intensity exercises. Muscle oxidative capacity (''k'') was assessed by near-infrared spectroscopy. Histochemical analysis, O<sub>2</sub> flux (''J''O<sub>2</sub>) by high-resolution respirometry, and quantification of key molecular markers of mitochondrial biogenesis and dynamics were performed in vastus lateralis biopsies. | ||
Pulmonary and cardiac functions were within normal range in all patients. | Pulmonary and cardiac functions were within normal range in all patients. VO<sub>2peak</sub> was lower in PASC than CTRL (24.7ยฑ5.0vs32.9ยฑ7.4mL*min<sup>-1</sup>*kg<sup>-1</sup>, respectively, P<.05). VO<sub>2</sub> kinetics was slower in PASC than CTRL (41ยฑ12vs30ยฑ9s<sup>-1</sup>, P<.05). ''k'' was lower in PASC than CTRL (1.54ยฑ0.49vs2.07ยฑ0.51min<sup>-1</sup>, P<.05). Citrate synthase, PGC1alfa and ''J''O<sub>2</sub> for mitochondrial complex II were significantly lower in PASC vs CTRL (all P<.05). | ||
In our cohort of patients with PASC, we showed limited exercise tolerance mainly due to "peripheral" determinants. Substantial reductions were observed for biomarkers of mitochondrial function, content, and biogenesis. PASC syndrome appears to negatively impact skeletal muscle function, although the disease is an heterogenous condition. | In our cohort of patients with PASC, we showed limited exercise tolerance mainly due to "peripheral" determinants. Substantial reductions were observed for biomarkers of mitochondrial function, content, and biogenesis. PASC syndrome appears to negatively impact skeletal muscle function, although the disease is an heterogenous condition. | ||
|keywords=LongCOVID, Mitochondria, Muscle oxidative metabolism, Muscle weakness, Myopathy | |||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Exercise physiology;nutrition;life style, Patients | ||
|diseases=Infectious | |||
|organism=Human | |||
|tissues=Skeletal muscle | |||
|preparations=Permeabilized tissue | |||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=N, S, NS, ROX | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2023-09 | |additional=2023-09 | ||
}} | }} |
Latest revision as of 17:57, 30 October 2023
Colosio M, Brocca L, Gatti M, Neri M, Crea E, Cadile F, Canepari M, Pellegrino MA, Polla B, Porcelli S, Bottinelli R (2023) Structural and functional impairments of skeletal muscle in patients with post-acute sequelae of SARS-CoV-2 infection. https://doi.org/10.1152/japplphysiol.00158.2023 |
ยป J Appl Physiol (1985) 135:4. PMID: 37675472 Open Access
Colosio Marta, Brocca Lorenza, Gatti Marco, Neri Marianna, Crea Emanuela, Cadile Francesca, Canepari Monica, Pellegrino Maria Antonietta, Polla Biagio, Porcelli Simone, Bottinelli Roberto (2023) J Appl Physiol (1985)
Abstract: Following acute COVID-19, a substantial proportion of patients showed symptoms and sequelae for several months, namely the post-acute sequelae of COVID-19 (PASC) syndrome. Major phenomena are exercise intolerance, muscle weakness and fatigue. We aimed to investigate the physiopathology of exercise intolerance in patients with PASC syndrome by structural and functional analyses of skeletal muscle.
At least 3 months after infection, non-hospitalized patients with PASC (n=11,ys:54ยฑ11; PASC) and patients without long-term symptoms (n=12,ys:49ยฑ9; CTRL) visited the laboratory on four non-consecutive days. Spirometry, lung diffusion capacity and quality of life were assessed at rest. Cardiopulmonary incremental exercise test was performed. Oxygen consumption (VO2) kinetics were determined by moderate-intensity exercises. Muscle oxidative capacity (k) was assessed by near-infrared spectroscopy. Histochemical analysis, O2 flux (JO2) by high-resolution respirometry, and quantification of key molecular markers of mitochondrial biogenesis and dynamics were performed in vastus lateralis biopsies.
Pulmonary and cardiac functions were within normal range in all patients. VO2peak was lower in PASC than CTRL (24.7ยฑ5.0vs32.9ยฑ7.4mL*min-1*kg-1, respectively, P<.05). VO2 kinetics was slower in PASC than CTRL (41ยฑ12vs30ยฑ9s-1, P<.05). k was lower in PASC than CTRL (1.54ยฑ0.49vs2.07ยฑ0.51min-1, P<.05). Citrate synthase, PGC1alfa and JO2 for mitochondrial complex II were significantly lower in PASC vs CTRL (all P<.05).
In our cohort of patients with PASC, we showed limited exercise tolerance mainly due to "peripheral" determinants. Substantial reductions were observed for biomarkers of mitochondrial function, content, and biogenesis. PASC syndrome appears to negatively impact skeletal muscle function, although the disease is an heterogenous condition. โข Keywords: LongCOVID, Mitochondria, Muscle oxidative metabolism, Muscle weakness, Myopathy โข Bioblast editor: Plangger M
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Patients
Pathology: Infectious
Organism: Human Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k
2023-09