Porter 2022 Abstract Bioblast: Difference between revisions
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{{Abstract | {{Abstract | ||
|title=Karavyraki M, Gnaiger E, Porter | |title=6.3. '''Β«10+5Β»''' [[File:Richie.jpg|left|100px|Porter Richard K]] Karavyraki M, Gnaiger E, <u>Porter Richard K</u> (2022) A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. '''Bioblast 2022: BEC Inaugural Conference.''' In: https://doi.org/10.26124/bec:2022-0001 <br>[[Karavyraki 2022 MitoFit|Β»''MitoFit Preprint''Β«]] [[File:WatchThePresentationYoutube_icon.jpg|200px|link=https://www.youtube.com/watch?v=GyS9ePicMlg&t=1925s|Β»''Watch the presentation''Β«]] | ||
|info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | |info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | ||
|authors=Karavyraki | |authors=Karavyraki Marilena, Gnaiger Erich, Porter Richard K | ||
|year=2022 | |year=2022 | ||
|event=Bioblast 2022 | |event=Bioblast 2022 | ||
|abstract=In an endeavour to understand the metabolic phenotype behind | |abstract=In an endeavour to understand the metabolic phenotype behind oral squamous cell carcinomas [1], we characterised the bioenergetic profile of a human tongue derived cancer cell line (SCC-4 cells) and compared this profile to a pre-cancerous dysplastic oral keratinocyte (DOK) cell line also derived from human tongue. The human SCC-4 cancer cells had greater mitochondrial density but lower mitochondrial O<sub>2</sub> flow per cell ''J''<sub>O<sub>2</sub></sub> than DOK cells. The lower cell ''J''<sub>O<sub>2</sub></sub> in SCC-4 cells can be partially explained by lower NADH-related enzymatic activity when compared to pre-cancerous DOK cells. In addition, SCC-4 cells have greater extracellular acidification rate (an index of glycolytic flux) when compared to DOK cells. In addition, treatment with recombinant human IL-6 (rhIL-6), known to drive anoikis resistance in SCC-4 cells but not DOK cells, impairs oxygen consumption in SCC-4 but not DOK cells, without affecting mitochondrial density. We conclude that SCC-4 cells have a less oxidative phenotype compared to DOK cells and that IL-6 attenuates mitochondrial function in SCC-4 cells while increasing glycolytic flux. | ||
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# Karavyraki M, Porter RK (2022) Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma. https://doi.org/10.1007/s12032-022-01664-5 | |||
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|keywords=Oral Squamous Cancer Cells, Mitochondria, Interleukin 6, Dysplastic oral keratinocytes, Oxygen consumption | |||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
}} | }} | ||
== Affiliations and support == | == Affiliations and support == | ||
Marilena Karavyraki | :::: Marilena Karavyraki<sup>1</sup>, Erich Gnaiger<sup>2</sup>, Richard K Porter<sup>1</sup> | ||
::::#School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland. | ::::# School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland - rkporter@tcd.ie | ||
::::#Oroboros Instruments, Innsbruck, Austria | ::::# Oroboros Instruments, Innsbruck, Austria | ||
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:::: Acknowledgements: Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 [[MitoEAGLE]] (2016-2021). | |||
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== List of abbreviations, terms and definitions - MitoPedia == | |||
{{Template:List of abbreviations, terms and definitions - MitoPedia}} | |||
{{Labeling | |||
|event=B2 | |||
|area=Respiration | |||
|diseases=Cancer | |||
|organism=Human | |||
|preparations=Permeabilized cells | |||
|enzymes=Complex I, Marker enzyme, TCA cycle and matrix dehydrogenases | |||
|topics=Aerobic glycolysis | |||
|couplingstates=LEAK, ROUTINE, ET | |||
|pathways=S, ROX | |||
|instruments=Oxygraph-2k | |||
|additional=Crabtree effect | |||
|articletype=Abstract | |||
}} |
Latest revision as of 08:40, 28 July 2022
6.3. Β«10+5Β» Karavyraki M, Gnaiger E, Porter Richard K (2022) A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 Β»MitoFit PreprintΒ« |
Link: Bioblast 2022: BEC Inaugural Conference
Karavyraki Marilena, Gnaiger Erich, Porter Richard K (2022)
Event: Bioblast 2022
In an endeavour to understand the metabolic phenotype behind oral squamous cell carcinomas [1], we characterised the bioenergetic profile of a human tongue derived cancer cell line (SCC-4 cells) and compared this profile to a pre-cancerous dysplastic oral keratinocyte (DOK) cell line also derived from human tongue. The human SCC-4 cancer cells had greater mitochondrial density but lower mitochondrial O2 flow per cell JO2 than DOK cells. The lower cell JO2 in SCC-4 cells can be partially explained by lower NADH-related enzymatic activity when compared to pre-cancerous DOK cells. In addition, SCC-4 cells have greater extracellular acidification rate (an index of glycolytic flux) when compared to DOK cells. In addition, treatment with recombinant human IL-6 (rhIL-6), known to drive anoikis resistance in SCC-4 cells but not DOK cells, impairs oxygen consumption in SCC-4 but not DOK cells, without affecting mitochondrial density. We conclude that SCC-4 cells have a less oxidative phenotype compared to DOK cells and that IL-6 attenuates mitochondrial function in SCC-4 cells while increasing glycolytic flux.
- Karavyraki M, Porter RK (2022) Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma. https://doi.org/10.1007/s12032-022-01664-5
β’ Keywords: Oral Squamous Cancer Cells, Mitochondria, Interleukin 6, Dysplastic oral keratinocytes, Oxygen consumption β’ Bioblast editor: Plangger M
Affiliations and support
- Marilena Karavyraki1, Erich Gnaiger2, Richard K Porter1
- School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland - [email protected]
- Oroboros Instruments, Innsbruck, Austria
- Marilena Karavyraki1, Erich Gnaiger2, Richard K Porter1
- Acknowledgements: Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 MitoEAGLE (2016-2021).
List of abbreviations, terms and definitions - MitoPedia
Labels: MiParea: Respiration
Pathology: Cancer
Organism: Human
Preparation: Permeabilized cells Enzyme: Complex I, Marker enzyme, TCA cycle and matrix dehydrogenases Regulation: Aerobic glycolysis Coupling state: LEAK, ROUTINE, ET Pathway: S, ROX HRR: Oxygraph-2k Event: B2 Crabtree effect