Porter 2022 Abstract Bioblast: Difference between revisions
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{{Abstract | {{Abstract | ||
|title=[[File:Richie.jpg|left|100px|Porter Richard K]] Karavyraki M, Gnaiger E, <u>Porter Richard K</u> (2022) A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. Bioblast 2022: BEC Inaugural Conference. | |title=[[File:Richie.jpg|left|100px|Porter Richard K]] Karavyraki M, Gnaiger E, <u>Porter Richard K</u> (2022) A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. Bioblast 2022: BEC Inaugural Conference. | ||
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# Karavyraki M, Porter RK (2022) Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma. https://doi.org/10.1007/s12032-022-01664-5 | # Karavyraki M, Porter RK (2022) Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma. https://doi.org/10.1007/s12032-022-01664-5 | ||
</small> | </small> | ||
|keywords=Oral Squamous Cancer Cells, Mitochondria, Interleukin 6, Dysplastic oral keratinocytes, Oxygen consumption | |keywords=Oral Squamous Cancer Cells, Mitochondria, Interleukin 6, Dysplastic oral keratinocytes, Oxygen consumption | ||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
}} | }} | ||
{{Labeling | |||
|area=Respiration | |||
|diseases=Cancer | |||
|organism=Human | |||
|preparations=Permeabilized cells | |||
|enzymes=Complex I, Marker enzyme, TCA cycle and matrix dehydrogenases | |||
|topics=Aerobic glycolysis | |||
|couplingstates=LEAK, ROUTINE, ET | |||
|pathways=S, ROX | |||
|additional=Crabtree effect | |||
|articletype=Abstract | |||
}} | |||
[[File:Bioblast2022 banner.jpg|link=Bioblast_2022]] | |||
== Affiliations and support == | == Affiliations and support == | ||
:::: Marilena | :::: Marilena Karavyraki1, Erich Gnaiger(2), Richard K Porter(1) | ||
::::# School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland - [email protected] | ::::# School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland - [email protected] | ||
::::# Oroboros Instruments, Innsbruck, Austria | ::::# Oroboros Instruments, Innsbruck, Austria | ||
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== List of abbreviations, terms and definitions - MitoPedia == | == List of abbreviations, terms and definitions - MitoPedia == | ||
{{Template:List of abbreviations, terms and definitions - MitoPedia}} | {{Template:List of abbreviations, terms and definitions - MitoPedia}} | ||
Revision as of 11:21, 2 June 2022
Karavyraki M, Gnaiger E, Porter Richard K (2022) A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. Bioblast 2022: BEC Inaugural Conference. |
Link: Bioblast 2022: BEC Inaugural Conference
Karavyraki Marilena, Gnaiger Erich, Porter Richard K (2022)
Event: Bioblast 2022
In an endeavour to understand the metabolic phenotype behind oral squamous cell carcinomas [1], we characterised the bioenergetic profile of a human tongue derived cancer cell line (SCC-4 cells) and compared this profile to a pre-cancerous dysplastic oral keratinocyte (DOK) cell line also derived from human tongue. The human SCC-4 cancer cells had greater mitochondrial density but lower mitochondrial O2 flow per cell JO2 than DOK cells. The lower cell JO2 in SCC-4 cells can be partially explained by lower NADH-related enzymatic activity when compared to pre-cancerous DOK cells. In addition, SCC-4 cells have greater extracellular acidification rate (an index of glycolytic flux) when compared to DOK cells. In addition, treatment with recombinant human IL-6 (rhIL-6), known to drive anoikis resistance in SCC-4 cells but not DOK cells, impairs oxygen consumption in SCC-4 but not DOK cells, without affecting mitochondrial density. We conclude that SCC-4 cells have a less oxidative phenotype compared to DOK cells and that IL-6 attenuates mitochondrial function in SCC-4 cells while increasing glycolytic flux.
- Karavyraki M, Porter RK (2022) Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma. https://doi.org/10.1007/s12032-022-01664-5
โข Keywords: Oral Squamous Cancer Cells, Mitochondria, Interleukin 6, Dysplastic oral keratinocytes, Oxygen consumption โข Bioblast editor: Plangger M
Labels: MiParea: Respiration Pathology: Cancer
Organism: Human
Preparation: Permeabilized cells Enzyme: Complex I, Marker enzyme, TCA cycle and matrix dehydrogenases Regulation: Aerobic glycolysis Coupling state: LEAK, ROUTINE, ET Pathway: S, ROX
Crabtree effect
Affiliations and support
- Marilena Karavyraki1, Erich Gnaiger(2), Richard K Porter(1)
- School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland - [email protected]
- Oroboros Instruments, Innsbruck, Austria
- Marilena Karavyraki1, Erich Gnaiger(2), Richard K Porter(1)
- Acknowledgements: Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 MitoEAGLE (2016-2021).