Liepinsh 2021 Free Radic Biol Med: Difference between revisions
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|title=Liepinsh E, Kuka J, Vilks K, Svalbe B, Stelfa G, Vilskersts R, Sevostjanovs E, Goldins NR, Groma V, Grinberga S, Plaas M, Makrecka-Kuka M, Dambrova M (2021) Low cardiac content of long-chain acylcarnitines in TMLHE knockout mice prevents ischaemia-reperfusion-induced mitochondrial and cardiac damage. Free Radic Biol Med [Epub ahead of print]. | |title=Liepinsh E, Kuka J, Vilks K, Svalbe B, Stelfa G, Vilskersts R, Sevostjanovs E, Goldins NR, Groma V, Grinberga S, Plaas M, Makrecka-Kuka M, Dambrova M (2021) Low cardiac content of long-chain acylcarnitines in TMLHE knockout mice prevents ischaemia-reperfusion-induced mitochondrial and cardiac damage. Free Radic Biol Med [Epub ahead of print]. | ||
|info=[https://pubmed.ncbi.nlm.nih.gov/34728372 PMID: 34728372 Open Access] | |info=[https://pubmed.ncbi.nlm.nih.gov/34728372 PMID: 34728372 Open Access] | ||
|authors=Liepinsh | |authors=Liepinsh Edgars, Kuka Janis, Vilks Karlis, Svalbe Baiba, Stelfa Gundega, Vilskersts Reinis, Sevostjanovs Eduards, Goldins Niks Ricards, Groma Valerija, Grinberga Solveiga, Plaas Mario, Makrecka-Kuka Marina, Dambrova Maija | ||
|year=2021 | |year=2021 | ||
|journal=Free Radic Biol Med | |journal=Free Radic Biol Med |
Revision as of 16:22, 10 November 2021
Liepinsh E, Kuka J, Vilks K, Svalbe B, Stelfa G, Vilskersts R, Sevostjanovs E, Goldins NR, Groma V, Grinberga S, Plaas M, Makrecka-Kuka M, Dambrova M (2021) Low cardiac content of long-chain acylcarnitines in TMLHE knockout mice prevents ischaemia-reperfusion-induced mitochondrial and cardiac damage. Free Radic Biol Med [Epub ahead of print]. |
Liepinsh Edgars, Kuka Janis, Vilks Karlis, Svalbe Baiba, Stelfa Gundega, Vilskersts Reinis, Sevostjanovs Eduards, Goldins Niks Ricards, Groma Valerija, Grinberga Solveiga, Plaas Mario, Makrecka-Kuka Marina, Dambrova Maija (2021) Free Radic Biol Med
Abstract: Increased tissue content of long-chain acylcarnitines may induce mitochondrial and cardiac damage by stimulating ROS production. N6-trimethyllysine dioxygenase (TMLD) is the first enzyme in the carnitine/acylcarnitine biosynthesis pathway. Inactivation of the TMLHE gene (TMLHE KO) in mice is expected to limit long-chain acylcarnitine synthesis and thus induce a cardio- and mitochondria-protective phenotype. TMLHE gene deletion in male mice lowered acylcarnitine concentrations in blood and cardiac tissues by up to 85% and decreased fatty acid oxidation by 30% but did not affect muscle and heart function in mice. Metabolome profile analysis revealed increased levels of polyunsaturated fatty acids (PUFAs) and a global shift in fatty acid content from saturated to unsaturated lipids. In the risk area of ischemic hearts in TMLHE KO mouse, the OXPHOS-dependent respiration rate and OXPHOS coupling efficiency were fully preserved. Additionally, the decreased long-chain acylcarnitine synthesis rate in TMLHE KO mice prevented ischaemia-reperfusion-induced ROS production in cardiac mitochondria. This was associated with a 39% smaller infarct size in the TMLHE KO mice. The arrest of the acylcarnitine biosynthesis pathway in TMLHE KO mice prevents ischaemia-reperfusion-induced damage in cardiac mitochondria and decreases infarct size. These results confirm that the decreased accumulation of ROS-increasing fatty acid metabolism intermediates prevents mitochondrial and cardiac damage during ischaemia-reperfusion.
โข Bioblast editor: Plangger M
Labels: MiParea: Respiration
HRR: Oxygraph-2k
2021-11