Sobotka 2015 Physiol Res

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Sobotka O, Drahota Z, Kučera O, Endlicher R, Rauchová H, Červinková Z (2015) The effect of α-tocopheryl succinate on succinate respiration in rat liver mitochondria. Physiol Res 64 Suppl 5:S609-15.

» PMID: 26674283 Open Access

Sobotka O, Drahota Z, Kucera O, Endlicher R, Rauchova H, Cervinkova Z (2015) Physiol Res

Abstract: We compared the effect of alpha-tocopheryl succinate (TOS) on succinate-dependent respiration in rat liver mitochondria, homogenate and permeabilized hepatocytes in both a coupled and uncoupled state. In isolated mitochondria, a significant inhibitory effect was observed at a concentration of 5 microM, in liver homogenate at 25 microM and in permeabilized hepatocytes at 50 microM. The inhibitory effect of TOS on succinate respiration in an uncoupled state was less pronounced than in a coupled state in all the experimental models tested. When the concentration dependence of the TOS inhibitory effect was tested, the most sensitive in both states were isolated mitochondria; the most resistant were permeabilized hepatocytes.

Keywords: Tocopheryl succinate, Complex II, Liver, Mitochondria, Homogenate, Hepatocytes

O2k-Network Lab: CZ Hradec Kralove Cervinkova Z, CZ Prague Houstek J


Labels: MiParea: Respiration, Pharmacology;toxicology 


Organism: Rat  Tissue;cell: Liver  Preparation: Permeabilized cells, Homogenate, Isolated mitochondria 


Coupling state: OXPHOS, ET  Pathway:HRR: Oxygraph-2k 

2016-03